LOCUS                 pEnT6P_Y        5380 bp ds-DNA   circular     12-SEP-2009
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     sig_peptide     202..231
                     /label=TY epitope
                     /ApEinfo_fwdcolor=#d6394b
                     /ApEinfo_revcolor=#d6394b
     sig_peptide     958..987
                     /label=TY epitope(1)
                     /ApEinfo_label=TY epitope
                     /ApEinfo_fwdcolor=#d6394b
                     /ApEinfo_revcolor=#d6394b
     rep_origin      4480..5162
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     CDS             join(238..927,929..951)
                     /label=YFP(no ATG)
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     CDS             3723..4382
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     CDS             1846..2442
                     /label=PURO
                     /ApEinfo_fwdcolor=#0064ff
                     /ApEinfo_revcolor=#0064ff
     5'UTR           4..192
                     /label=Aldolase 5'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     3'UTR           1000..1702
                     /label=Aldolase 3'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     5'UTR           1710..1840
                     /label=Actin 5'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     3'UTR           2447..2855
                     /label=Actin 3'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
ORIGIN
        1 GGGTGCTCAA GCTGTGTAGC GCACGCGTTT CCTTACATAT TTCTCTAACA GGCACGGAAG
       61 CCTAACAAAT ACACTTGGCT TATTTTTTTG CCCCCTCATG TCTTGTACAA ATATTTGCGA
      121 TAGCTTAGCT ATCAGCCACA TTAATCAAAC AAGTATACCA ACAAGCCCGA AAACATAAAC
      181 TCAACTGCAA CGAAGCTTAT GGAGGTCCAT ACTAACCAAG ATCCACTTGA CACTAGTGTG
      241 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
      301 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
      361 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
      421 ACCACCTTCG GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT GAAGCAGCAC
      481 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
      541 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
      601 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
      661 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC
      721 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC
      781 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
      841 AGCTACCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG
      901 GAGTTCGTGA CCGCCGCCGG GATCACTcTC GGCATGGACG AGCTGTACAA GtctagAGAG
      961 GTCCATACTA ACCAAGATCC ACTTGACTAA GGATCCTGCC CATTTAGTTG GCTTTTCCCT
     1021 TGTCTCGTGT CTTTTCCGTG GAAAGGTTCC CGGAGTAATC TGATGGCACA GCAGGGAGGT
     1081 GCGCCTGCAG GTTGGTTAGG AAGGGGGGAT GATGTAAAAG AAGAAAATGG GGGGATATCT
     1141 TATGTTTAAG AGGATAAATA ATGTGAAGGG GCTTTATATG CTTGCTTGGT TGTCTCGTTG
     1201 GTGCATGGGG AATCTGCATG TTTGCTTTGG AGCACGCGTG GTACATCAAT GGGATCATAT
     1261 CTTGCTGCCT CCCGCAGCTC ACCGTGCGAG CTGCCGGGAC CCCGTTTCTC ATAGGCGTGT
     1321 GCACCTTCCG TCTGAGACCT GTAAATGGTT AAAAAGGAAT ATATAATTAC CTTTTGAAAA
     1381 GTGGTAAAAA ACGAATATAT CTTTTTTTTT GGTTTTAATA CGCTCTTTTG TGTGTATGAG
     1441 AGGAATAAAT AAGTGTGTGT GTGTGTGTGT GTTGTTGTTG TTGAAAAAAT GAAGGACACG
     1501 AAAGCGCTCA TTAAGGGTGG GGATACACCA GGCATAATTA TCCGAAAATA TTTTGATGCA
     1561 CCAAATAAGT GAACATACTG ACGAAATCAA AGAGTTGGAG GATGGATAGG GAGGCCTCAT
     1621 TGGCAGTGGT AAATTGATTT GACTTGAATA TGATACCCTC TTACTGTTTC ATTGCCACTA
     1681 CAAGTTGGTT TCCTTCCCCT GCAGGCATGC AAGCTAGGGG CACAGCAAGG TCTTCTGAAA
     1741 TTCATGTTTT TTTTTTTTTT ACTCTGCATT GCAGTCTCCG CTCTTATTTA GTTTTGCTTT
     1801 ACGTAAGGTC TCGTTGCTGC CATAAAATAA GCTCTAGTGG AATTCATGAC CGAGTACAAG
     1861 CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC CCTCGCCGCC
     1921 GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGATC CAGACCGCCA CATCGAGCGG
     1981 GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG
     2041 GTCGCGGACG ACGGCGCAGC AGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG
     2101 GCGGTGTTCG CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG
     2161 CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG
     2221 GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC
     2281 CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC
     2341 CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC
     2401 GAAGGACCGC GCACTTGGTG CATGACCCGC AAGCCCGGTG CCTAACCATG Ggttgtgtgg
     2461 ccaaatttgt tctgtagttg ctgtgagttg acacggctag tgcttatgat tttcctcgcg
     2521 cgtggtgcct gtactcagcc ctatgcctta tatgcaacac atttacgtac agcgcacaag
     2581 aggagagaag atcacttgaa gataataaat atagggttgt aggcatcttg tttaactcaa
     2641 attttctcgc cttggtgtgt cgacatgatt gaaatagtgc caccagttgt gtttgatgcg
     2701 tttgttatct atgcagcatt gcacagcaag gtcttctgaa attcatgttt ttttttttta
     2761 ctctgcattg cagtctccgc tcttatttag ttttgcttta cgtaaggtct cgttactgcc
     2821 ataaaataat gtcggacgag gaacaaactg ctatTCTAGC CAGATCCTCT ACGCCGGACG
     2881 CATCGTGGCC GGCATCACCG GCGCCACAGG TGCGGTTGCT GGCGCCTATA TCGCCGACAT
     2941 CACCGATGGG GAAGATCGGG CTCGCCACTT CGGGCTCATG AGCGCTTGTT TCGGCGTGGG
     3001 TATGGTGGCA GGCCCCGTGG CCGGGGGACT GTTGGGCGCC ATCTCCTTGC ACCATTCCTT
     3061 GCGGCGGCGG TGCTCAACGG CCTCAACCTA CTACTGGGCT GCTTCCTAAT GCAGGAGTCG
     3121 CATAAGGGAG AGCGTCGATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT
     3181 TAAGCCAGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC
     3241 CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT
     3301 CACCGTCATC ACCGAAACGC GCGAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG
     3361 TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC
     3421 GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC
     3481 AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT
     3541 TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG
     3601 AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG
     3661 AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA
     3721 TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC
     3781 AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG
     3841 TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA
     3901 CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC
     3961 TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG
     4021 AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA
     4081 CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA
     4141 TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG
     4201 GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG
     4261 CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG
     4321 CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT
     4381 GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT
     4441 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC
     4501 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG
     4561 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG
     4621 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA
     4681 GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA
     4741 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA
     4801 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC
     4861 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA
     4921 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA
     4981 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC
     5041 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC
     5101 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG
     5161 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT
     5221 CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA
     5281 GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA
     5341 AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG  
//
