LOCUS                 pEnT6B_Y        5173 bp ds-DNA   circular     12-SEP-2009
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     sig_peptide     202..231
                     /label=TY epitope
                     /ApEinfo_fwdcolor=#d6394b
                     /ApEinfo_revcolor=#d6394b
     sig_peptide     958..987
                     /label=TY epitope(1)
                     /ApEinfo_label=TY epitope
                     /ApEinfo_fwdcolor=#d6394b
                     /ApEinfo_revcolor=#d6394b
     rep_origin      4273..4955
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     CDS             join(238..927,929..951)
                     /label=YFP(no ATG)
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     CDS             3516..4175
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     CDS             1846..2235
                     /label=BLAST
                     /ApEinfo_fwdcolor=#0064ff
                     /ApEinfo_revcolor=#0064ff
     5'UTR           4..192
                     /label=Aldolase 5'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     3'UTR           1000..1702
                     /label=Aldolase 3'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     5'UTR           1710..1840
                     /label=Actin 5'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     3'UTR           2240..2648
                     /label=Actin 3'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
ORIGIN
        1 GGGTGCTCAA GCTGTGTAGC GCACGCGTTT CCTTACATAT TTCTCTAACA GGCACGGAAG
       61 CCTAACAAAT ACACTTGGCT TATTTTTTTG CCCCCTCATG TCTTGTACAA ATATTTGCGA
      121 TAGCTTAGCT ATCAGCCACA TTAATCAAAC AAGTATACCA ACAAGCCCGA AAACATAAAC
      181 TCAACTGCAA CGAAGCTTAT GGAGGTCCAT ACTAACCAAG ATCCACTTGA CACTAGTGTG
      241 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
      301 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
      361 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
      421 ACCACCTTCG GCTACGGCCT GCAGTGCTTC GCCCGCTACC CCGACCACAT GAAGCAGCAC
      481 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
      541 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
      601 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
      661 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC
      721 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC
      781 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
      841 AGCTACCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG
      901 GAGTTCGTGA CCGCCGCCGG GATCACTcTC GGCATGGACG AGCTGTACAA GtctagAGAG
      961 GTCCATACTA ACCAAGATCC ACTTGACTAA GGATCCTGCC CATTTAGTTG GCTTTTCCCT
     1021 TGTCTCGTGT CTTTTCCGTG GAAAGGTTCC CGGAGTAATC TGATGGCACA GCAGGGAGGT
     1081 GCGCCTGCAG GTTGGTTAGG AAGGGGGGAT GATGTAAAAG AAGAAAATGG GGGGATATCT
     1141 TATGTTTAAG AGGATAAATA ATGTGAAGGG GCTTTATATG CTTGCTTGGT TGTCTCGTTG
     1201 GTGCATGGGG AATCTGCATG TTTGCTTTGG AGCACGCGTG GTACATCAAT GGGATCATAT
     1261 CTTGCTGCCT CCCGCAGCTC ACCGTGCGAG CTGCCGGGAC CCCGTTTCTC ATAGGCGTGT
     1321 GCACCTTCCG TCTGAGACCT GTAAATGGTT AAAAAGGAAT ATATAATTAC CTTTTGAAAA
     1381 GTGGTAAAAA ACGAATATAT CTTTTTTTTT GGTTTTAATA CGCTCTTTTG TGTGTATGAG
     1441 AGGAATAAAT AAGTGTGTGT GTGTGTGTGT GTTGTTGTTG TTGAAAAAAT GAAGGACACG
     1501 AAAGCGCTCA TTAAGGGTGG GGATACACCA GGCATAATTA TCCGAAAATA TTTTGATGCA
     1561 CCAAATAAGT GAACATACTG ACGAAATCAA AGAGTTGGAG GATGGATAGG GAGGCCTCAT
     1621 TGGCAGTGGT AAATTGATTT GACTTGAATA TGATACCCTC TTACTGTTTC ATTGCCACTA
     1681 CAAGTTGGTT TCCTTCCCCT GCAGGCATGC AAGCTAGGGG CACAGCAAGG TCTTCTGAAA
     1741 TTCATGTTTT TTTTTTTTTT ACTCTGCATT GCAGTCTCCG CTCTTATTTA GTTTTGCTTT
     1801 ACGTAAGGTC TCGTTGCTGC CATAAAATAA GCTCTAGTGG AATTCATGCC TTTGTCTCAA
     1861 GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC CATCTCTGAA
     1921 GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC TGGTGTCAAT
     1981 GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC TGCTGCTGCT
     2041 GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG GGGCATCTTG
     2101 AGCCCCTGCG GACGGTGTCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT CAAAGCGATA
     2161 GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT GCCCTCTGGT
     2221 TATGTGTGGG AGGGCTAACC ATGGgttgtg tggccaaatt tgttctgtag ttgctgtgag
     2281 ttgacacggc tagtgcttat gattttcctc gcgcgtggtg cctgtactca gccctatgcc
     2341 ttatatgcaa cacatttacg tacagcgcac aagaggagag aagatcactt gaagataata
     2401 aatatagggt tgtaggcatc ttgtttaact caaattttct cgccttggtg tgtcgacatg
     2461 attgaaatag tgccaccagt tgtgtttgat gcgtttgtta tctatgcagc attgcacagc
     2521 aaggtcttct gaaattcatg tttttttttt ttactctgca ttgcagtctc cgctcttatt
     2581 tagttttgct ttacgtaagg tctcgttact gccataaaat aatgtcggac gaggaacaaa
     2641 ctgctatTCT AGCCAGATCC TCTACGCCGG ACGCATCGTG GCCGGCATCA CCGGCGCCAC
     2701 AGGTGCGGTT GCTGGCGCCT ATATCGCCGA CATCACCGAT GGGGAAGATC GGGCTCGCCA
     2761 CTTCGGGCTC ATGAGCGCTT GTTTCGGCGT GGGTATGGTG GCAGGCCCCG TGGCCGGGGG
     2821 ACTGTTGGGC GCCATCTCCT TGCACCATTC CTTGCGGCGG CGGTGCTCAA CGGCCTCAAC
     2881 CTACTACTGG GCTGCTTCCT AATGCAGGAG TCGCATAAGG GAGAGCGTCG ATATGGTGCA
     2941 CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC
     3001 CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA
     3061 CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGAC
     3121 GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT
     3181 AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT
     3241 AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT
     3301 ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG
     3361 CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG
     3421 AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC
     3481 TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT
     3541 GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT
     3601 ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA
     3661 TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT
     3721 TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG
     3781 ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG
     3841 AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG
     3901 AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG
     3961 CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG
     4021 CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC
     4081 GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA
     4141 TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT
     4201 ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC
     4261 TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG
     4321 ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT
     4381 GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC
     4441 CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC
     4501 TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG
     4561 CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT
     4621 TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT
     4681 GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC
     4741 TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA
     4801 GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA
     4861 GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG
     4921 GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT
     4981 GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA
     5041 CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG
     5101 TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA
     5161 TTCATTAATG CAG    
//
