LOCUS                 pEnT6B_C        5176 bp ds-DNA   circular     12-SEP-2009
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     sig_peptide     202..231
                     /label=TY epitope
                     /ApEinfo_fwdcolor=#d6394b
                     /ApEinfo_revcolor=#d6394b
     sig_peptide     961..990
                     /label=TY epitope(1)
                     /ApEinfo_label=TY epitope
                     /ApEinfo_fwdcolor=#d6394b
                     /ApEinfo_revcolor=#d6394b
     rep_origin      4276..4958
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     CDS             238..954
                     /label=CFP
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
     CDS             3519..4178
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     CDS             1849..2238
                     /label=BLAST
                     /ApEinfo_fwdcolor=#0064ff
                     /ApEinfo_revcolor=#0064ff
     5'UTR           4..192
                     /label=Aldolase 5'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     3'UTR           1003..1705
                     /label=Aldolase 3'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     5'UTR           1713..1843
                     /label=Actin 5'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     3'UTR           2243..2651
                     /label=Actin 3'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
ORIGIN
        1 GGGTGCTCAA GCTGTGTAGC GCACGCGTTT CCTTACATAT TTCTCTAACA GGCACGGAAG
       61 CCTAACAAAT ACACTTGGCT TATTTTTTTG CCCCCTCATG TCTTGTACAA ATATTTGCGA
      121 TAGCTTAGCT ATCAGCCACA TTAATCAAAC AAGTATACCA ACAAGCCCGA AAACATAAAC
      181 TCAACTGCAA CGAAGCTTAT GGAGGTCCAT ACTAACCAAG ATCCACTTGA CACTAGTATG
      241 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC
      301 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC
      361 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC
      421 GTGACCACCC TGACCTGGGG CGTGCAGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG
      481 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC
      541 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG
      601 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG
      661 CTGGAGTACA ACGCCATCAG CGACAACGTC TATATCACCG CCGACAAGCA GAAGAACGGC
      721 ATCAAGGCCA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC
      781 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC
      841 CTGAGCACCC AGTCCAAGCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG
      901 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGtctagA
      961 GAGGTCCATA CTAACCAAGA TCCACTTGAC TAAGGATCCT GCCCATTTAG TTGGCTTTTC
     1021 CCTTGTCTCG TGTCTTTTCC GTGGAAAGGT TCCCGGAGTA ATCTGATGGC ACAGCAGGGA
     1081 GGTGCGCCTG CAGGTTGGTT AGGAAGGGGG GATGATGTAA AAGAAGAAAA TGGGGGGATA
     1141 TCTTATGTTT AAGAGGATAA ATAATGTGAA GGGGCTTTAT ATGCTTGCTT GGTTGTCTCG
     1201 TTGGTGCATG GGGAATCTGC ATGTTTGCTT TGGAGCACGC GTGGTACATC AATGGGATCA
     1261 TATCTTGCTG CCTCCCGCAG CTCACCGTGC GAGCTGCCGG GACCCCGTTT CTCATAGGCG
     1321 TGTGCACCTT CCGTCTGAGA CCTGTAAATG GTTAAAAAGG AATATATAAT TACCTTTTGA
     1381 AAAGTGGTAA AAAACGAATA TATCTTTTTT TTTGGTTTTA ATACGCTCTT TTGTGTGTAT
     1441 GAGAGGAATA AATAAGTGTG TGTGTGTGTG TGTGTTGTTG TTGTTGAAAA AATGAAGGAC
     1501 ACGAAAGCGC TCATTAAGGG TGGGGATACA CCAGGCATAA TTATCCGAAA ATATTTTGAT
     1561 GCACCAAATA AGTGAACATA CTGACGAAAT CAAAGAGTTG GAGGATGGAT AGGGAGGCCT
     1621 CATTGGCAGT GGTAAATTGA TTTGACTTGA ATATGATACC CTCTTACTGT TTCATTGCCA
     1681 CTACAAGTTG GTTTCCTTCC CCTGCAGGCA TGCAAGCTAG GGGCACAGCA AGGTCTTCTG
     1741 AAATTCATGT TTTTTTTTTT TTTACTCTGC ATTGCAGTCT CCGCTCTTAT TTAGTTTTGC
     1801 TTTACGTAAG GTCTCGTTGC TGCCATAAAA TAAGCTCTAG TGGAATTCAT GCCTTTGTCT
     1861 CAAGAAGAAT CCACCCTCAT TGAAAGAGCA ACGGCTACAA TCAACAGCAT CCCCATCTCT
     1921 GAAGACTACA GCGTCGCCAG CGCAGCTCTC TCTAGCGACG GCCGCATCTT CACTGGTGTC
     1981 AATGTATATC ATTTTACTGG GGGACCTTGT GCAGAACTCG TGGTGCTGGG CACTGCTGCT
     2041 GCTGCGGCAG CTGGCAACCT GACTTGTATC GTCGCGATCG GAAATGAGAA CAGGGGCATC
     2101 TTGAGCCCCT GCGGACGGTG TCGACAGGTG CTTCTCGATC TGCATCCTGG GATCAAAGCG
     2161 ATAGTGAAGG ACAGTGATGG ACAGCCGACG GCAGTTGGGA TTCGTGAATT GCTGCCCTCT
     2221 GGTTATGTGT GGGAGGGCTA ACCATGGgtt gtgtggccaa atttgttctg tagttgctgt
     2281 gagttgacac ggctagtgct tatgattttc ctcgcgcgtg gtgcctgtac tcagccctat
     2341 gccttatatg caacacattt acgtacagcg cacaagagga gagaagatca cttgaagata
     2401 ataaatatag ggttgtaggc atcttgttta actcaaattt tctcgccttg gtgtgtcgac
     2461 atgattgaaa tagtgccacc agttgtgttt gatgcgtttg ttatctatgc agcattgcac
     2521 agcaaggtct tctgaaattc atgttttttt tttttactct gcattgcagt ctccgctctt
     2581 atttagtttt gctttacgta aggtctcgtt actgccataa aataatgtcg gacgaggaac
     2641 aaactgctat TCTAGCCAGA TCCTCTACGC CGGACGCATC GTGGCCGGCA TCACCGGCGC
     2701 CACAGGTGCG GTTGCTGGCG CCTATATCGC CGACATCACC GATGGGGAAG ATCGGGCTCG
     2761 CCACTTCGGG CTCATGAGCG CTTGTTTCGG CGTGGGTATG GTGGCAGGCC CCGTGGCCGG
     2821 GGGACTGTTG GGCGCCATCT CCTTGCACCA TTCCTTGCGG CGGCGGTGCT CAACGGCCTC
     2881 AACCTACTAC TGGGCTGCTT CCTAATGCAG GAGTCGCATA AGGGAGAGCG TCGATATGGT
     2941 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA
     3001 CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG
     3061 TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA
     3121 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT
     3181 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT
     3241 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT
     3301 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT
     3361 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG
     3421 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA
     3481 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC
     3541 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC
     3601 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG
     3661 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA
     3721 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG
     3781 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG
     3841 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG
     3901 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG
     3961 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG
     4021 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT
     4081 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC
     4141 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT
     4201 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA
     4261 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT
     4321 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT
     4381 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC
     4441 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC
     4501 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC
     4561 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG
     4621 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT
     4681 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG
     4741 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG
     4801 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT
     4861 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG
     4921 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT
     4981 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA
     5041 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT
     5101 CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC
     5161 CGATTCATTA ATGCAG    
//
