LOCUS                  pEnT5_G        6313 bp ds-DNA   circular     12-SEP-2009
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     sig_peptide     202..231
                     /label=TY epitope
                     /ApEinfo_fwdcolor=#d6394b
                     /ApEinfo_revcolor=#d6394b
     sig_peptide     967..996
                     /label=TY epitope(1)
                     /ApEinfo_label=TY epitope
                     /ApEinfo_fwdcolor=#d6394b
                     /ApEinfo_revcolor=#d6394b
     terminator      3483..3622
                     /label=T7 terminator
                     /ApEinfo_fwdcolor=#937644
                     /ApEinfo_revcolor=#937644
     rep_origin      5409..6091
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     CDS             238..951
                     /label=GFP(no ATG)
                     /ApEinfo_fwdcolor=green
                     /ApEinfo_revcolor=green
     CDS             4652..5311
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     CDS             1848..2870
                     /label=HYG
                     /ApEinfo_fwdcolor=#0064ff
                     /ApEinfo_revcolor=#0064ff
     3'UTR           2902..3184
                     /label=Actin 3'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     3'UTR           3200..3482
                     /label=Actin 3'(1)
                     /ApEinfo_label=Actin 3'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     5'UTR           4..192
                     /label=Aldolase 5'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     3'UTR           1009..1711
                     /label=Aldolase 3'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     5'UTR           1712..1847
                     /label=Actin 5'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
ORIGIN
        1 GGGTGCTCAA GCTGTGTAGC GCACGCGTTT CCTTACATAT TTCTCTAACA GGCACGGAAG
       61 CCTAACAAAT ACACTTGGCT TATTTTTTTG CCCCCTCATG TCTTGTACAA ATATTTGCGA
      121 TAGCTTAGCT ATCAGCCACA TTAATCAAAC AAGTATACCA ACAAGCCCGA AAACAGAAAC
      181 TCAACTGCAA CGAAGCTTAT GGAGGTCCAT ACTAACCAAG ATCCACTTGA CACTAGTGTG
      241 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
      301 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
      361 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
      421 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC
      481 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
      541 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
      601 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
      661 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC
      721 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC
      781 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
      841 AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG
      901 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GCGGTACCGT
      961 TCTAGAGAGG TCCATACTAA CCAAGATCCA CTTGACTAAG GATCCTGCCC ATTTAGTTGG
     1021 CTTTTCCCTT GTCTCGTGTC TTTTCCGTGG AAAGGTTCCC GGAGTAATCT GATGGCACAG
     1081 CAGGGAGGTG CGCCTGCAGG TTGGTTAGGA AGGGGGGATG ATGTAAAAGA AGAAAATGGG
     1141 GGGATATCTT ATGTTTAAGA GGATAAATAA TGTGAAGGGG CTTTATATGC TTGCTTGGTT
     1201 GTCTCGTTGG TGCATGGGGA ATCTGCATGT TTGCTTTGGA GCACGCGTGG TACATCAATG
     1261 GGATCATATC TTGCTGCCTC CCGCAGTCAC CGTGCGGAGC TGCCGGTGCC CCGTTTCTCA
     1321 TAGGCGTGTG CACCTTCCGT CTGAGACCTG TAAATGGTTA AAAAGGAATA TATAATTACC
     1381 TTTTGAAAAG TGGTAAAAAA CGAATATATG TTTTTTTTTG GTTTTAATAC GCTCTTTTGT
     1441 GTGTATGAGA GGAATAAATA AGTGTGTGTG TGTGTGTGTG TTGTTGTTGT TGAAAAAATG
     1501 AAGGACACGA AAGCGCTCAT TAAGGGTGGG GATACACCAG GCATAATTAT CCGAAAATAT
     1561 TTTGATGCAC CAAATAAGTG AACATACTGA CGAAATCAAA GAGTTGGAGG ATGGATAGGG
     1621 AGGCCTCATT GGCAGTGGTA AATTGATTTG ACTTGAATAT GATACCCTCT TACTGTTTCA
     1681 TTGCCACTAC AAGTTGGTTT CCTTCCCCTG CAGGCATGCA GGCTAGGGCA CAGCAAGGTC
     1741 TTCTGAAATT CATGTTTTTT TTTTTTTTAC TCTGCATTGC AGTCTCCGCT CTTATTTAGT
     1801 TTTGCTTTAC GTAAGGTCTC GTTGCTGCCA TAAAATAAGC TCTAGTGATG AAAAAGCCTG
     1861 AACTCACCGC GACGTCTGTC GAGAAGTTTC TGATCGAAAA GTTCGACAGC GTCTCCGACC
     1921 TGATGCAGCT CTCGGAGGGC GAAGAATCTC GTGCTTTCAG CTTCGATGTA GGAGGGCGTG
     1981 GATATGTCCT GCGGGTAAAT AGCTGCGCCG ATGGTTTCTA CAAAGATCGT TATGTTTATC
     2041 GGCACTTTGC ATCGGCCGCG CTCCCGATTC CGGAAGTGCT TGACATTGGG GAATTCAGCG
     2101 AGAGCCTGAC CTATTGCATC TCCCGCCGTG CACAGGGTGT CACGTTGCAA GACCTGCCTG
     2161 AAACCGAACT GCCCGCTGTT CTGCAGCCGG TCGCGGAGGC CATGGATGCG ATCGCTGCGG
     2221 CCGATCTTAG CCAGACGAGC GGGTTCGGCC CATTCGGACC GCAAGGAATC GGTCAATACA
     2281 CTACATGGCG TGATTTCATA TGCGCGATTG CTGATCCCCA TGTGTATCAC TGGCAAACTG
     2341 TGATGGACGA CACCGTCAGT GCGTCCGTCG CGCAGGCTCT CGATGAGCTG ATGCTTTGGG
     2401 CCGAGGACTG CCCCGAAGTC CGGCACCTCG TGCACGCGGA TTTCGGCTCC AACAATGTCC
     2461 TGACGGACAA TGGCCGCATA ACAGCGGTCA TTGACTGGAG CGAGGCGATG TTCGGGGATT
     2521 CCCAATACGA GGTCGCCAAC ATCTTCTTCT GGAGGCCGTG GTTGGCTTGT ATGGAGCAGC
     2581 AGACGCGCTA CTTCGAGCGG AGGCATCCGG AGCTTGCAGG ATCGCCGCGG CTCCGGGCGT
     2641 ATATGCTCCG CATTGGTCTT GACCAACTCT ATCAGAGCTT GGTTGACGGC AATTTCGATG
     2701 ATGCAGCTTG GGCGCAGGGT CGATGCGACG CAATCGTCCG ATCCGGAGCC GGGACTGTCG
     2761 GGCGTACACA AATCGCCCGC AGAAGCGCGG CCGTCTGGAC CGATGGCTGT GTAGAAGTAC
     2821 TCGCCGATAG TGGAAACCGA CGCCCCAGCA CTCGTCCGAG GGCAAAGGAA TAGAGTAGAT
     2881 GCCGACCGGG ATCGATCCCC CGATCCTAAC ACCGGGTTGT GTGGCCAAAA TTGTTCTGTA
     2941 GTCGCTGTGA GTTGACACGG CTAGTGCTTA TGATTTTCCT CGCGTGTGGT GCCTGTACTC
     3001 AGCCCTATGC CTTATTTGCA ACACATTTAC GTACAGCGCA CAAGAGAAGA GAAGATCACT
     3061 TGAAGATAAT AAATATAGGG TTGTAGGCAT CTTGTTTAAC TCAAATTTTC TCGTCTTGGT
     3121 GTGTCGACAT GATTGAAATA GTGCCACCAG TTGTGTTTGA TGCGTTTGTT ATCTATGCAG
     3181 TATTCTGCAA GGCCTCGGAG ATCCTAACAC CGGGTTGTGT GGCCAAAATT GTTCTGTAGT
     3241 CGCTGTGAGT TGACACGGCT AGTGCTTATG ATTTTCCTCG CGTGTGGTGC CTGTACTCAG
     3301 CCCTATGCCT TATTTGCAAC ACATTTACGT ACAGCGCACA AGAGAAGAGA AGATCACTTG
     3361 AAGATAATAA ATATAGGGTT GTAGGCATCT TGTTTAACTC AAATTTTCTC GTCTTGGTGT
     3421 GTCGACATGA TTGAAATAGT GCCACCAGTT GTGTTTGATG CGTTTGTTAT CTATGCAGTA
     3481 TTCTGCAGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC
     3541 AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG
     3601 GAGAACTATA TCCGGATAGG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG
     3661 CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT
     3721 TTTTGCTGAA AGGAGAACTA TATCCGGATT GGGCCCTAgG GGATAACAGG GTAATGCATG
     3781 CAAGCTAGCC AGATCCTCTA CGCCGGACGC ATCGTGGCCG GCATCACCGG CGCCACAGGT
     3841 GCGGTTGCTG GCGCCTATAT CGCCGACATC ACCGATGGGG AAGATCGGGC TCGCCACTTC
     3901 GGGCTCATGA GCGCTTGTTT CGGCGTGGGT ATGGTGGCAG GCCCCGTGGC CGGGGGACTG
     3961 TTGGGCGCCA TCTCCTTGCA CCATTCCTTG CGGCGGCGGT GCTCAACGGC CTCAACCTAC
     4021 TACTGGGCTG CTTCCTAATG CAGGAGTCGC ATAAGGGAGA GCGTCGAtAT GGTGCACTCT
     4081 CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC
     4141 TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT
     4201 CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA
     4261 GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC
     4321 GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT
     4381 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG
     4441 AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC
     4501 ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA
     4561 TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA
     4621 GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG
     4681 CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC
     4741 TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC
     4801 AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT
     4861 TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA
     4921 TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG
     4981 TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT
     5041 ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG
     5101 ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG
     5161 TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT
     5221 CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC
     5281 TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT
     5341 ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT
     5401 TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC
     5461 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT
     5521 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC
     5581 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT
     5641 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT
     5701 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA
     5761 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC
     5821 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG
     5881 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT
     5941 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC
     6001 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG
     6061 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC
     6121 TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC
     6181 CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG
     6241 CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA
     6301 TTAATGCAGT CGA    
//
