LOCUS                  pEnT5_C        6307 bp ds-DNA   circular     12-SEP-2009
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     sig_peptide     202..231
                     /label=TY epitope
                     /ApEinfo_fwdcolor=#d6394b
                     /ApEinfo_revcolor=#d6394b
     sig_peptide     961..990
                     /label=TY epitope(1)
                     /ApEinfo_label=TY epitope
                     /ApEinfo_fwdcolor=#d6394b
                     /ApEinfo_revcolor=#d6394b
     terminator      3477..3616
                     /label=T7 terminator
                     /ApEinfo_fwdcolor=#937644
                     /ApEinfo_revcolor=#937644
     rep_origin      5403..6085
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     CDS             238..954
                     /label=CFP
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
     CDS             4646..5305
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
     CDS             1842..2864
                     /label=HYG
                     /ApEinfo_fwdcolor=#0064ff
                     /ApEinfo_revcolor=#0064ff
     3'UTR           2896..3178
                     /label=Actin 3'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     3'UTR           3194..3476
                     /label=Actin 3'(1)
                     /ApEinfo_label=Actin 3'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     5'UTR           4..192
                     /label=Aldolase 5'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     3'UTR           1003..1705
                     /label=Aldolase 3'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     5'UTR           1706..1841
                     /label=Actin 5'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
ORIGIN
        1 GGGTGCTCAA GCTGTGTAGC GCACGCGTTT CCTTACATAT TTCTCTAACA GGCACGGAAG
       61 CCTAACAAAT ACACTTGGCT TATTTTTTTG CCCCCTCATG TCTTGTACAA ATATTTGCGA
      121 TAGCTTAGCT ATCAGCCACA TTAATCAAAC AAGTATACCA ACAAGCCCGA AAACAGAAAC
      181 TCAACTGCAA CGAAGCTTAT GGAGGTCCAT ACTAACCAAG ATCCACTTGA CACTAGTATG
      241 GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC
      301 GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC
      361 AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC
      421 GTGACCACCC TGACCTGGGG CGTGCAGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG
      481 CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC
      541 AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG
      601 AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG
      661 CTGGAGTACA ACGCCATCAG CGACAACGTC TATATCACCG CCGACAAGCA GAAGAACGGC
      721 ATCAAGGCCA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC
      781 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC
      841 CTGAGCACCC AGTCCAAGCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG
      901 CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGtctagA
      961 GAGGTCCATA CTAACCAAGA TCCACTTGAC TAAGGATCCT GCCCATTTAG TTGGCTTTTC
     1021 CCTTGTCTCG TGTCTTTTCC GTGGAAAGGT TCCCGGAGTA ATCTGATGGC ACAGCAGGGA
     1081 GGTGCGCCTG CAGGTTGGTT AGGAAGGGGG GATGATGTAA AAGAAGAAAA TGGGGGGATA
     1141 TCTTATGTTT AAGAGGATAA ATAATGTGAA GGGGCTTTAT ATGCTTGCTT GGTTGTCTCG
     1201 TTGGTGCATG GGGAATCTGC ATGTTTGCTT TGGAGCACGC GTGGTACATC AATGGGATCA
     1261 TATCTTGCTG CCTCCCGCAG TCACCGTGCG GAGCTGCCGG TGCCCCGTTT CTCATAGGCG
     1321 TGTGCACCTT CCGTCTGAGA CCTGTAAATG GTTAAAAAGG AATATATAAT TACCTTTTGA
     1381 AAAGTGGTAA AAAACGAATA TATGTTTTTT TTTGGTTTTA ATACGCTCTT TTGTGTGTAT
     1441 GAGAGGAATA AATAAGTGTG TGTGTGTGTG TGTGTTGTTG TTGTTGAAAA AATGAAGGAC
     1501 ACGAAAGCGC TCATTAAGGG TGGGGATACA CCAGGCATAA TTATCCGAAA ATATTTTGAT
     1561 GCACCAAATA AGTGAACATA CTGACGAAAT CAAAGAGTTG GAGGATGGAT AGGGAGGCCT
     1621 CATTGGCAGT GGTAAATTGA TTTGACTTGA ATATGATACC CTCTTACTGT TTCATTGCCA
     1681 CTACAAGTTG GTTTCCTTCC CCTGCAGGCA TGCAGGCTAG GGCACAGCAA GGTCTTCTGA
     1741 AATTCATGTT TTTTTTTTTT TTACTCTGCA TTGCAGTCTC CGCTCTTATT TAGTTTTGCT
     1801 TTACGTAAGG TCTCGTTGCT GCCATAAAAT AAGCTCTAGT GATGAAAAAG CCTGAACTCA
     1861 CCGCGACGTC TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGCGTCTCC GACCTGATGC
     1921 AGCTCTCGGA GGGCGAAGAA TCTCGTGCTT TCAGCTTCGA TGTAGGAGGG CGTGGATATG
     1981 TCCTGCGGGT AAATAGCTGC GCCGATGGTT TCTACAAAGA TCGTTATGTT TATCGGCACT
     2041 TTGCATCGGC CGCGCTCCCG ATTCCGGAAG TGCTTGACAT TGGGGAATTC AGCGAGAGCC
     2101 TGACCTATTG CATCTCCCGC CGTGCACAGG GTGTCACGTT GCAAGACCTG CCTGAAACCG
     2161 AACTGCCCGC TGTTCTGCAG CCGGTCGCGG AGGCCATGGA TGCGATCGCT GCGGCCGATC
     2221 TTAGCCAGAC GAGCGGGTTC GGCCCATTCG GACCGCAAGG AATCGGTCAA TACACTACAT
     2281 GGCGTGATTT CATATGCGCG ATTGCTGATC CCCATGTGTA TCACTGGCAA ACTGTGATGG
     2341 ACGACACCGT CAGTGCGTCC GTCGCGCAGG CTCTCGATGA GCTGATGCTT TGGGCCGAGG
     2401 ACTGCCCCGA AGTCCGGCAC CTCGTGCACG CGGATTTCGG CTCCAACAAT GTCCTGACGG
     2461 ACAATGGCCG CATAACAGCG GTCATTGACT GGAGCGAGGC GATGTTCGGG GATTCCCAAT
     2521 ACGAGGTCGC CAACATCTTC TTCTGGAGGC CGTGGTTGGC TTGTATGGAG CAGCAGACGC
     2581 GCTACTTCGA GCGGAGGCAT CCGGAGCTTG CAGGATCGCC GCGGCTCCGG GCGTATATGC
     2641 TCCGCATTGG TCTTGACCAA CTCTATCAGA GCTTGGTTGA CGGCAATTTC GATGATGCAG
     2701 CTTGGGCGCA GGGTCGATGC GACGCAATCG TCCGATCCGG AGCCGGGACT GTCGGGCGTA
     2761 CACAAATCGC CCGCAGAAGC GCGGCCGTCT GGACCGATGG CTGTGTAGAA GTACTCGCCG
     2821 ATAGTGGAAA CCGACGCCCC AGCACTCGTC CGAGGGCAAA GGAATAGAGT AGATGCCGAC
     2881 CGGGATCGAT CCCCCGATCC TAACACCGGG TTGTGTGGCC AAAATTGTTC TGTAGTCGCT
     2941 GTGAGTTGAC ACGGCTAGTG CTTATGATTT TCCTCGCGTG TGGTGCCTGT ACTCAGCCCT
     3001 ATGCCTTATT TGCAACACAT TTACGTACAG CGCACAAGAG AAGAGAAGAT CACTTGAAGA
     3061 TAATAAATAT AGGGTTGTAG GCATCTTGTT TAACTCAAAT TTTCTCGTCT TGGTGTGTCG
     3121 ACATGATTGA AATAGTGCCA CCAGTTGTGT TTGATGCGTT TGTTATCTAT GCAGTATTCT
     3181 GCAAGGCCTC GGAGATCCTA ACACCGGGTT GTGTGGCCAA AATTGTTCTG TAGTCGCTGT
     3241 GAGTTGACAC GGCTAGTGCT TATGATTTTC CTCGCGTGTG GTGCCTGTAC TCAGCCCTAT
     3301 GCCTTATTTG CAACACATTT ACGTACAGCG CACAAGAGAA GAGAAGATCA CTTGAAGATA
     3361 ATAAATATAG GGTTGTAGGC ATCTTGTTTA ACTCAAATTT TCTCGTCTTG GTGTGTCGAC
     3421 ATGATTGAAA TAGTGCCACC AGTTGTGTTT GATGCGTTTG TTATCTATGC AGTATTCTGC
     3481 AGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC
     3541 TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGAAC
     3601 TATATCCGGA TAGGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG
     3661 CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC
     3721 TGAAAGGAGA ACTATATCCG GATTGGGCCC TAgGGGATAA CAGGGTAATG CATGCAAGCT
     3781 AGCCAGATCC TCTACGCCGG ACGCATCGTG GCCGGCATCA CCGGCGCCAC AGGTGCGGTT
     3841 GCTGGCGCCT ATATCGCCGA CATCACCGAT GGGGAAGATC GGGCTCGCCA CTTCGGGCTC
     3901 ATGAGCGCTT GTTTCGGCGT GGGTATGGTG GCAGGCCCCG TGGCCGGGGG ACTGTTGGGC
     3961 GCCATCTCCT TGCACCATTC CTTGCGGCGG CGGTGCTCAA CGGCCTCAAC CTACTACTGG
     4021 GCTGCTTCCT AATGCAGGAG TCGCATAAGG GAGAGCGTCG AtATGGTGCA CTCTCAGTAC
     4081 AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC CCGCTGACGC
     4141 GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG
     4201 GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGAC GAAAGGGCCT
     4261 CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG
     4321 TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC
     4381 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG
     4441 GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG
     4501 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT
     4561 GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT
     4621 TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT
     4681 ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA
     4741 TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG
     4801 AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC
     4861 AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC
     4921 TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC
     4981 CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC
     5041 TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT
     5101 TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG
     5161 TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT
     5221 TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT
     5281 AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA
     5341 GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA
     5401 TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA
     5461 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC
     5521 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT
     5581 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC
     5641 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT
     5701 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG
     5761 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC
     5821 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG
     5881 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC
     5941 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG
     6001 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT
     6061 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC
     6121 TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA
     6181 GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     6241 AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG
     6301 CAGTCGA     
//
