LOCUS                pDex577_G        6768 bp ds-DNA   circular     28-FEB-2010
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(2614..2633)
                     /label=T7
                     /ApEinfo_fwdcolor=#d6d4ff
                     /ApEinfo_revcolor=#fbc0fc
     primer_bind     757..774
                     /label=SP6
                     /ApEinfo_fwdcolor=#d6d4ff
                     /ApEinfo_revcolor=#fbc0fc
     primer_bind     complement(3414..3433)
                     /label=pEnT-seq2
                     /ApEinfo_fwdcolor=#d6d4ff
                     /ApEinfo_revcolor=#fbc0fc
     repeat_region   13..544
                     /label=177-targeting
                     /ApEinfo_fwdcolor=#d6d4ff
                     /ApEinfo_revcolor=#fbc0fc
     sig_peptide     complement(1514..1543)
                     /label=TY epitope
                     /ApEinfo_fwdcolor=#d6394b
                     /ApEinfo_revcolor=#d6394b
     sig_peptide     complement(2279..2308)
                     /label=TY epitope(1)
                     /ApEinfo_label=TY epitope
                     /ApEinfo_fwdcolor=#d6394b
                     /ApEinfo_revcolor=#d6394b
     rep_origin      5868..6550
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     CDS             complement(1559..2272)
                     /label=GFP
                     /ApEinfo_fwdcolor=green
                     /ApEinfo_revcolor=green
     CDS             5111..5770
                     /label=AmpR
                     /ApEinfo_fwdcolor=#d8b6b0
                     /ApEinfo_revcolor=#d8b6b0
     promoter        3111..3401
                     /label=rDNA promoter
                     /ApEinfo_fwdcolor=cornflower blue
                     /ApEinfo_revcolor=cornflower blue
     CDS             3530..3901
                     /label=BLEO
                     /ApEinfo_fwdcolor=#0064ff
                     /ApEinfo_revcolor=#0064ff
     5'UTR           complement(2313..2405)
                     /label=EP1 5'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     3'UTR           3961..4243
                     /label=Actin 3'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     3'UTR           complement(812..1504)
                     /label=Aldolase 3'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     protein_bind    complement(2438..2456)
                     /label=Tet-O
                     /ApEinfo_fwdcolor=#b6897e
                     /ApEinfo_revcolor=#b6897e
     protein_bind    complement(2459..2477)
                     /label=Tet-O(1)
                     /ApEinfo_label=Tet-O
                     /ApEinfo_fwdcolor=#b6897e
                     /ApEinfo_revcolor=#b6897e
     protein_bind    complement(2590..2608)
                     /label=Tet-O(2)
                     /ApEinfo_label=Tet-O
                     /ApEinfo_fwdcolor=#b6897e
                     /ApEinfo_revcolor=#b6897e
     terminator      2822..2961
                     /label=T7 terminator
                     /ApEinfo_fwdcolor=#937644
                     /ApEinfo_revcolor=#937644
ORIGIN
        1 GAATTCGAGC TCTTTAATGG TCCTTATACG AACGAATATT AAACAATGCG CAGTTAACGC
       61 TATTATACAC AATAACTTTT AATGTCTGCC ATATTAATTA CAAGTGTGCA ACATTAAATA
      121 CAAGTGTGTA ACGTTAATTT GCAAGTTTGA AACAATGTTC TTTAGTGTTT AATGTGTGCA
      181 ACAAAGCTAT TTAATGGTCC TTATACGAAC GAATATTAAA CAATGCGCAG TTAACGCTAT
      241 TATACACAAT AACTTTTAAT GTGTGCCATA TTAATTACGG ATCGCGGCCG CGATCCGTGT
      301 GCAACATTAA ATACAAGTGT GTAACATTAA TTTGCAAGTT TGCAACAATG TTCTTTAGTG
      361 TTTAATGGGT GCAACAAAGC TATTTAATGG TCCTTATACG AACGAATATT AAACAATCCG
      421 CAGTTAACGC TATTATACAC AATAACTTTT AATGTGTCCC ATATTAATTA CAAGTGTGCA
      481 ACATTAAATA CAAGTGTGTA ACATTAATTT GCAAGTTTGC AACAATGTTC TTTAGTGTTT
      541 AATGGGTACC ATTGGTTCGC TGACCATTTC CGGGTGCGGG ACGGCGTTAC CAGAAACTCA
      601 GAAGGTTCGT CCAACCAAAC CGACTCTGAC GGCAGTTTAC GAGAGAGATG ATAGGGTCTG
      661 CTTCAGTAAG CCAGATGCTA CACAATTAGG CTTGTACATA TTGTCGTTAG AACGCGGCTA
      721 CAATTAATAC ATAACCTTAT GTATCATACA CATACGATTT AGGTGACACT ATAGAATACA
      781 AGCTAGCCTG CATGCCTGCA GGGGAAGGAA ACCAACTTGT AGTGGCAATG AAACAGTAAG
      841 AGGGTATCAT ATTCAAGTCA AATCAATTTA CCACTGCCAA TGAGGCCTCC CTATCCATCC
      901 TCCAACTCTT TGATTTCGTC AGTATGTTCA CTTATTTGGT GCATCAAAAT ATTTTCGGAT
      961 AATTATGCCT GGTGTATCCC CACCCTTAAT GAGCGCTTTC GTGTCCTTCA TTTTTTCAAC
     1021 AACAACAACA CACACACACA CACACACTTA TTTATTCCTC TCATACACAC AAAAGAGCGT
     1081 ATTAAAACCA AAAAAAAACA TATATTCGTT TTTTACCACT TTTCAAAAGG TAATTATATA
     1141 TTCCTTTTTA ACCATTTACA GGTCTCAGAC GGAAGGTGCA CACGCCTATG AGAAACGGGG
     1201 CACCNGGCAG CTCCGCACGG TGACTGCGGG AGGCAGCAAG ATATGATCCC ATTGATGTAC
     1261 CACGCGTGCT CCAAAGCAAA CATGCAGATT CCCCATGCAC CAACGAGACA ACCAAGCAAG
     1321 CATATAAAGC CCCTTCACAT TATTTATCCT CTTAAACATA AGATATCCCC CCATTTTCTT
     1381 CTTTTACATC ATCCCCCCTT CCTAACCAAC CTGCAGGCGC ACCTCCCTGC TGTGCCATCA
     1441 GATTACTCCG GGAACCTTTC CACGGAAAAG ACACGAGACA AGGGAAAAGC CAACTAAATG
     1501 GGCAGGATCC TTAGTCAAGT GGATCTTGGT TAGTATGGAC CTCTCTAGAA CGGTACCGCT
     1561 TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA
     1621 TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT
     1681 TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA
     1741 GCTGCACGCT GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG ATGCCGTTCT
     1801 TCTGCTTGTC GGCCATGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC
     1861 CCAGGATGTT GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG
     1921 TGTCGCCCTC GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA
     1981 TGGTGCGCTC CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA
     2041 TGTGGTCGGG GTAGCGGCTG AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG
     2101 GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTAGG
     2161 TGGCATCGCC CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA
     2221 GCTCGACCAG GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ACACTAGTGT
     2281 CAAGTGGATC TTGGTTAGTA TGGACCTCCA TAAGCTTGTG AATTTTACTT TTTGGTGTAA
     2341 TTGAAGTCTT CAAGTTGAAA AAAATGGGGA AAAGGAAAAA AAACTCGAAG GCGCGTGCAG
     2401 CCCGGGACGA GAACATGTTA AACTCAGTAC TCAGGGATCT CTATCACTGA TAGGGAGATC
     2461 TCTATCACTG ATAGGGAGAT CCCTACCCGA TAACCCCTAT TTTCAATGCA CGCCCACCCA
     2521 GAGACACGGA ATAGCATAAA ACAACCCCAT CACTGCCACA AAATGGACTC GAGGGGGGGC
     2581 CCGGGGAGAT CTCTATCACT GATAGGGAGA TCTCCCTATA GTGAGTCGTA TTAATCAGCC
     2641 GGTGTGTTGC CAAAGACATT CTGTATAATT TTCCAACTAC CCTAACCTGA TTCACTCATC
     2701 GTTGAGCATT TGTTCTTCTA CTTTTTGACC GCAAGTGTCG TTATTAGTAT TCGGTCCTCC
     2761 TCGACATGAT TGAAATAGTG CCACCAGTTG TGTTTGATGC GTTTGTTATC TATGCAGTAT
     2821 TCTGCAGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA
     2881 ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG
     2941 AGAACTATAT CCGGATAGGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC
     3001 CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT
     3061 TTTGCTGAAA GGAGAACTAT ATCCGGATTG GGCCCCTGCA GGCATGCAAG CTAGCTTTCC
     3121 ACCCAGCGCG GGTGCATTCT GGCTCTTATA TATACTTATT GTCATGACAG AGTATATTGT
     3181 ACTGTGTTGA TAAGGGACGG GTAACTGTAT TGAAGAGCCG ATGCTTTTGA CATGTTAGAT
     3241 ATAATATGTT TTATTGTAAA GTCAATACAA CACACAATAG GATAATAATG ATAAAGTTAA
     3301 AAAAGTATAT ATAGTAATAG AAATATATCT TATATAGGAA AGATTAAGCA GTAAAAGTAG
     3361 CGCTTACGGC GTACGGAGCA GGAGAGCAAC TGACCGCTCT CAGACCCGGG CACAGCAAGG
     3421 TCTTCTGAAA TTCATGTTTT TTTTTTTTTT ACTCTGCATT GCAGTCTCCG CTCTTATTTA
     3481 GTTTTGCTTT ACGTAAGGTC TCGTTGCTGC CATAAAATAA GCTATCACCA TGGCCAAGTT
     3541 GACCAGTGCC GTTCCGGTGC TCACCGCGCG CGACGTCGCC GGAGCGGTCG AGTTCTGGAC
     3601 CGACCGGCTC GGGTTCTCCC GGGACTTCGT GGAGGACGAC TTCGCCGGTG TGGTCCGGGA
     3661 CGACGTGACC CTGTTCATCA GCGCGGTCCA GGACCAGGTG GTGCCGGACA ACACCCTGGC
     3721 CTGGGTGTGG GTGCGCGGCC TGGACGAGCT GTACGCCGAG TGGTCGGAGG TCGTGTCCAC
     3781 GAACTTCCGG GACGCCTCCG GGCCGGCCAT GACCGAGATC GGCGAGCAGC CGTGGGGGCG
     3841 GGAGTTCGCC CTGCGCGACC CGGCCGGCAA CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA
     3901 CTGACCGACG CCGACCAACA CCGCCGGTCC GACGCGGCCC GACGGGTCCG AGGCCTCGGA
     3961 GATCCTAACA CCGGGTTGTG TGGCCAAAAT TGTTCTGTAG TCGCTGTGAG TTGACACGGC
     4021 TAGTGCTTAT GATTTTCCTC GCGTGTGGTG CCTGTACTCA GCCCTATGCC TTATTTGCAA
     4081 CACATTTACG TACAGCGCAC AAGAGAAGAG AAGATCACTT GAAGATAATA AATATAGGGT
     4141 TGTAGGCATC TTGTTTAACT CAAATTTTCT CGTCTTGGTG TGTCGACATG ATTGAAATAG
     4201 TGCCACCAGT TGTGTTTGAT GCGTTTGTTA TCTATGCAGT ATTCCTAGCC AGATCCTCTA
     4261 CGCCGGACGC ATCGTGGCCG GCATCACCGG CGCCACAGGT GCGGTTGCTG GCGCCTATAT
     4321 CGCCGACATC ACCGATGGGG AAGATCGGGC TCGCCACTTC GGGCTCATGA GCGCTTGTTT
     4381 CGGCGTGGGT ATGGTGGCAG GCCCCGTGGC CGGGGGACTG TTGGGCGCCA TCTCCTTGCA
     4441 CCATTCCTTG CGGCGGCGGT GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG
     4501 CAGGAGTCGC ATAAGGGAGA GCGTCGAATG GTGCACTCTC AGTACAATCT GCTCTGATGC
     4561 CGCATAGTTA AGCCAGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG
     4621 TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA
     4681 GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGACGAAAG GGCCTCGTGA TACGCCTATT
     4741 TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG
     4801 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT
     4861 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT
     4921 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC
     4981 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG
     5041 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG
     5101 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA
     5161 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA
     5221 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC
     5281 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC
     5341 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG
     5401 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC
     5461 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA
     5521 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT
     5581 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT
     5641 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG
     5701 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT
     5761 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT
     5821 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT
     5881 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC
     5941 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT
     6001 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG
     6061 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA
     6121 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC
     6181 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA
     6241 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC
     6301 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA
     6361 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG
     6421 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG
     6481 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG
     6541 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC
     6601 TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC
     6661 TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC
     6721 AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAG 
//
