LOCUS                pDex577_C        6762 bp ds-DNA   circular     28-FEB-2010
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(2608..2627)
                     /label=T7
                     /ApEinfo_fwdcolor=#d6d4ff
                     /ApEinfo_revcolor=#fbc0fc
     primer_bind     757..774
                     /label=SP6
                     /ApEinfo_fwdcolor=#d6d4ff
                     /ApEinfo_revcolor=#fbc0fc
     primer_bind     complement(3408..3427)
                     /label=pEnT-seq2
                     /ApEinfo_fwdcolor=#d6d4ff
                     /ApEinfo_revcolor=#fbc0fc
     repeat_region   13..544
                     /label=177-targeting
                     /ApEinfo_fwdcolor=#d6d4ff
                     /ApEinfo_revcolor=#fbc0fc
     sig_peptide     complement(1514..1543)
                     /label=TY epitope
                     /ApEinfo_fwdcolor=#d6394b
                     /ApEinfo_revcolor=#d6394b
     sig_peptide     complement(2273..2302)
                     /label=TY epitope(1)
                     /ApEinfo_label=TY epitope
                     /ApEinfo_fwdcolor=#d6394b
                     /ApEinfo_revcolor=#d6394b
     rep_origin      5862..6544
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
     CDS             complement(1550..2266)
                     /label=CFP
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
     CDS             5105..5764
                     /label=AmpR
                     /ApEinfo_fwdcolor=#d8b6b0
                     /ApEinfo_revcolor=#d8b6b0
     promoter        3105..3395
                     /label=rDNA promoter
                     /ApEinfo_fwdcolor=cornflower blue
                     /ApEinfo_revcolor=cornflower blue
     CDS             3524..3895
                     /label=BLEO
                     /ApEinfo_fwdcolor=#0064ff
                     /ApEinfo_revcolor=#0064ff
     5'UTR           complement(2307..2399)
                     /label=EP1 5'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     3'UTR           3955..4237
                     /label=Actin 3'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     3'UTR           complement(812..1504)
                     /label=Aldolase 3'
                     /ApEinfo_fwdcolor=#bfbfbf
                     /ApEinfo_revcolor=#bfbfbf
     protein_bind    complement(2432..2450)
                     /label=Tet-O
                     /ApEinfo_fwdcolor=#b6897e
                     /ApEinfo_revcolor=#b6897e
     protein_bind    complement(2453..2471)
                     /label=Tet-O(1)
                     /ApEinfo_label=Tet-O
                     /ApEinfo_fwdcolor=#b6897e
                     /ApEinfo_revcolor=#b6897e
     protein_bind    complement(2584..2602)
                     /label=Tet-O(2)
                     /ApEinfo_label=Tet-O
                     /ApEinfo_fwdcolor=#b6897e
                     /ApEinfo_revcolor=#b6897e
     terminator      2816..2955
                     /label=T7 terminator
                     /ApEinfo_fwdcolor=#937644
                     /ApEinfo_revcolor=#937644
ORIGIN
        1 GAATTCGAGC TCTTTAATGG TCCTTATACG AACGAATATT AAACAATGCG CAGTTAACGC
       61 TATTATACAC AATAACTTTT AATGTCTGCC ATATTAATTA CAAGTGTGCA ACATTAAATA
      121 CAAGTGTGTA ACGTTAATTT GCAAGTTTGA AACAATGTTC TTTAGTGTTT AATGTGTGCA
      181 ACAAAGCTAT TTAATGGTCC TTATACGAAC GAATATTAAA CAATGCGCAG TTAACGCTAT
      241 TATACACAAT AACTTTTAAT GTGTGCCATA TTAATTACGG ATCGCGGCCG CGATCCGTGT
      301 GCAACATTAA ATACAAGTGT GTAACATTAA TTTGCAAGTT TGCAACAATG TTCTTTAGTG
      361 TTTAATGGGT GCAACAAAGC TATTTAATGG TCCTTATACG AACGAATATT AAACAATCCG
      421 CAGTTAACGC TATTATACAC AATAACTTTT AATGTGTCCC ATATTAATTA CAAGTGTGCA
      481 ACATTAAATA CAAGTGTGTA ACATTAATTT GCAAGTTTGC AACAATGTTC TTTAGTGTTT
      541 AATGGGTACC ATTGGTTCGC TGACCATTTC CGGGTGCGGG ACGGCGTTAC CAGAAACTCA
      601 GAAGGTTCGT CCAACCAAAC CGACTCTGAC GGCAGTTTAC GAGAGAGATG ATAGGGTCTG
      661 CTTCAGTAAG CCAGATGCTA CACAATTAGG CTTGTACATA TTGTCGTTAG AACGCGGCTA
      721 CAATTAATAC ATAACCTTAT GTATCATACA CATACGATTT AGGTGACACT ATAGAATACA
      781 AGCTAGCCTG CATGCCTGCA GGGGAAGGAA ACCAACTTGT AGTGGCAATG AAACAGTAAG
      841 AGGGTATCAT ATTCAAGTCA AATCAATTTA CCACTGCCAA TGAGGCCTCC CTATCCATCC
      901 TCCAACTCTT TGATTTCGTC AGTATGTTCA CTTATTTGGT GCATCAAAAT ATTTTCGGAT
      961 AATTATGCCT GGTGTATCCC CACCCTTAAT GAGCGCTTTC GTGTCCTTCA TTTTTTCAAC
     1021 AACAACAACA CACACACACA CACACACTTA TTTATTCCTC TCATACACAC AAAAGAGCGT
     1081 ATTAAAACCA AAAAAAAACA TATATTCGTT TTTTACCACT TTTCAAAAGG TAATTATATA
     1141 TTCCTTTTTA ACCATTTACA GGTCTCAGAC GGAAGGTGCA CACGCCTATG AGAAACGGGG
     1201 CACCsGGCAG CTCCGCACGG TGACTGCGGG AGGCAGCAAG ATATGATCCC ATTGATGTAC
     1261 CACGCGTGCT CCAAAGCAAA CATGCAGATT CCCCATGCAC CAACGAGACA ACCAAGCAAG
     1321 CATATAAAGC CCCTTCACAT TATTTATCCT CTTAAACATA AGATATCCCC CCATTTTCTT
     1381 CTTTTACATC ATCCCCCCTT CCTAACCAAC CTGCAGGCGC ACCTCCCTGC TGTGCCATCA
     1441 GATTACTCCG GGAACCTTTC CACGGAAAAG ACACGAGACA AGGGAAAAGC CAACTAAATG
     1501 GGCAGGATCC TTAGTCAAGT GGATCTTGGT TAGTATGGAC CTCTctagaC TTGTACAGCT
     1561 CGTCCATGCC GAGAGTGATC CCGGCGGCGG TCACGAACTC CAGCAGGACC ATGTGATCGC
     1621 GCTTCTCGTT GGGGTCTTTG CTCAGCTTGG ACTGGGTGCT CAGGTAGTGG TTGTCGGGCA
     1681 GCAGCACGGG GCCGTCGCCG ATGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC
     1741 TGCCGTCCTC GATGTTGTGG CGGATCTTGA AGTTGGCCTT GATGCCGTTC TTCTGCTTGT
     1801 CGGCGGTGAT ATAGACGTTG TCGCTGATGG CGTTGTACTC CAGCTTGTGC CCCAGGATGT
     1861 TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA GCTCGATGCG GTTCACCAGG GTGTCGCCCT
     1921 CGAACTTCAC CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC CTTGAAGAAG ATGGTGCGCT
     1981 CCTGGACGTA GCCTTCGGGC ATGGCGGACT TGAAGAAGTC GTGCTGCTTC ATGTGGTCGG
     2041 GGTAGCGGGC GAAGCACTGC ACGCCCCAGG TCAGGGTGGT CACGAGGGTG GGCCAGGGCA
     2101 CGGGCAGCTT GCCGGTGGTG CAGATGAACT TCAGGGTCAG CTTGCCGTAG GTGGCATCGC
     2161 CCTCGCCCTC GCCGGACACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC AGCTCGACCA
     2221 GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACCATACTA GTGTCAAGTG
     2281 GATCTTGGTT AGTATGGACC TCCATAAGCT TGTGAATTTT ACTTTTTGGT GTAATTGAAG
     2341 TCTTCAAGTT GAAAAAAATG GGGAAAAGGA AAAAAAACTC GAAGGCGCGT GCAGCCCGGG
     2401 ACGAGAACAT GTTAAACTCA GTACTCAGGG ATCTCTATCA CTGATAGGGA GATCTCTATC
     2461 ACTGATAGGG AGATCCCTAC CCGATAACCC CTATTTTCAA TGCACGCCCA CCCAGAGACA
     2521 CGGAATAGCA TAAAACAACC CCATCACTGC CACAAAATGG ACTCGAGGGG GGGCCCGGGG
     2581 AGATCTCTAT CACTGATAGG GAGATCTCCC TATAGTGAGT CGTATTAATC AGCCGGTGTG
     2641 TTGCCAAAGA CATTCTGTAT AATTTTCCAA CTACCCTAAC CTGATTCACT CATCGTTGAG
     2701 CATTTGTTCT TCTACTTTTT GACCGCAAGT GTCGTTATTA GTATTCGGTC CTCCTCGACA
     2761 TGATTGAAAT AGTGCCACCA GTTGTGTTTG ATGCGTTTGT TATCTATGCA GTATTCTGCA
     2821 GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT
     2881 AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGAACT
     2941 ATATCCGGAT AGGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC
     3001 TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT
     3061 GAAAGGAGAA CTATATCCGG ATTGGGCCCC TGCAGGCATG CAAGCTAGCT TTCCACCCAG
     3121 CGCGGGTGCA TTCTGGCTCT TATATATACT TATTGTCATG ACAGAGTATA TTGTACTGTG
     3181 TTGATAAGGG ACGGGTAACT GTATTGAAGA GCCGATGCTT TTGACATGTT AGATATAATA
     3241 TGTTTTATTG TAAAGTCAAT ACAACACACA ATAGGATAAT AATGATAAAG TTAAAAAAGT
     3301 ATATATAGTA ATAGAAATAT ATCTTATATA GGAAAGATTA AGCAGTAAAA GTAGCGCTTA
     3361 CGGCGTACGG AGCAGGAGAG CAACTGACCG CTCTCAGACC CGGGCACAGC AAGGTCTTCT
     3421 GAAATTCATG TTTTTTTTTT TTTTACTCTG CATTGCAGTC TCCGCTCTTA TTTAGTTTTG
     3481 CTTTACGTAA GGTCTCGTTG CTGCCATAAA ATAAGCTATC ACCATGGCCA AGTTGACCAG
     3541 TGCCGTTCCG GTGCTCACCG CGCGCGACGT CGCCGGAGCG GTCGAGTTCT GGACCGACCG
     3601 GCTCGGGTTC TCCCGGGACT TCGTGGAGGA CGACTTCGCC GGTGTGGTCC GGGACGACGT
     3661 GACCCTGTTC ATCAGCGCGG TCCAGGACCA GGTGGTGCCG GACAACACCC TGGCCTGGGT
     3721 GTGGGTGCGC GGCCTGGACG AGCTGTACGC CGAGTGGTCG GAGGTCGTGT CCACGAACTT
     3781 CCGGGACGCC TCCGGGCCGG CCATGACCGA GATCGGCGAG CAGCCGTGGG GGCGGGAGTT
     3841 CGCCCTGCGC GACCCGGCCG GCAACTGCGT GCACTTCGTG GCCGAGGAGC AGGACTGACC
     3901 GACGCCGACC AACACCGCCG GTCCGACGCG GCCCGACGGG TCCGAGGCCT CGGAGATCCT
     3961 AACACCGGGT TGTGTGGCCA AAATTGTTCT GTAGTCGCTG TGAGTTGACA CGGCTAGTGC
     4021 TTATGATTTT CCTCGCGTGT GGTGCCTGTA CTCAGCCCTA TGCCTTATTT GCAACACATT
     4081 TACGTACAGC GCACAAGAGA AGAGAAGATC ACTTGAAGAT AATAAATATA GGGTTGTAGG
     4141 CATCTTGTTT AACTCAAATT TTCTCGTCTT GGTGTGTCGA CATGATTGAA ATAGTGCCAC
     4201 CAGTTGTGTT TGATGCGTTT GTTATCTATG CAGTATTCCT AGCCAGATCC TCTACGCCGG
     4261 ACGCATCGTG GCCGGCATCA CCGGCGCCAC AGGTGCGGTT GCTGGCGCCT ATATCGCCGA
     4321 CATCACCGAT GGGGAAGATC GGGCTCGCCA CTTCGGGCTC ATGAGCGCTT GTTTCGGCGT
     4381 GGGTATGGTG GCAGGCCCCG TGGCCGGGGG ACTGTTGGGC GCCATCTCCT TGCACCATTC
     4441 CTTGCGGCGG CGGTGCTCAA CGGCCTCAAC CTACTACTGG GCTGCTTCCT AATGCAGGAG
     4501 TCGCATAAGG GAGAGCGTCG AATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA
     4561 GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT
     4621 CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT
     4681 TTCACCGTCA TCACCGAAAC GCGCGAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA
     4741 GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT
     4801 GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG
     4861 ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA
     4921 TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC
     4981 AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT
     5041 CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC
     5101 AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG
     5161 GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC
     5221 AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT
     5281 AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA
     5341 GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC
     5401 GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC
     5461 AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT
     5521 AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC
     5581 TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC
     5641 AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA
     5701 GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA
     5761 TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT
     5821 TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA
     5881 ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG
     5941 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC
     6001 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG
     6061 CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA
     6121 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC
     6181 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC
     6241 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA
     6301 CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG
     6361 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT
     6421 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA
     6481 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC
     6541 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT
     6601 ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG
     6661 CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG
     6721 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AG 
//
